C100-Pro Automated Cell Counter

C100-Pro Automated Cell Counter

A Real Time-Saver Speeds up Your Research

RWD automated cell counter is a reliable cell counting device with highly intelligent analysis software and excellent microscopy optics structure.

In order to meet the needs of cell therapy and biopharma, the data management and software system of C100-Pro fully conforms to FDA 21 CFR Part 11.

RWD C100-Pro Automatic Cell Counter Realizes Analysis With Multiple Application Scenarios

Cell Count Analysis: Suitable for counting cells or particles with a diameter of 4-60μm, such as most cell lines, stem cells, primary cells, etc.

Cell Viability Analysis: When testing cell viability in trypan blue mode, after staining, live cells will be circled in green, and dead cells will be circled in red.
 

Cell Size Analysis: In cell research, cell size is a key feature, which is often measured in cell transfection, drug testing and cell viability.

After counting, it will count the average diameter of the cells in the sample and automatically generate a histogram.
 

Cell Fluorescence Analysis:
 

Detect Cell Transfection Efficiency (GFP, RFP):
When transfecting cells with the target gene, the transfection efficiency needs to be evaluated. In general, the transfected plasmid has the sequence encoding the fluorescent protein as the reporter gene, so the successfully transfected cell should be positive for the fluorescent protein. In addition, using a fluorescence microscope or a flow cytometer is a commonly used method for transfection efficiency analysis in laboratories, but both have the disadvantages of complicated operation and high cost. Using the fluorescence analysis function of C100, equipped with the corresponding fluorescence module, can realize the analysis of the expression of GFP, RFP, and other fluorescent proteins, and automatically count them, so as to quickly, simply, and efficiently obtain the positive rate of target gene expression.
 

Cell Fluorescence Analysis:

Cell Viability Identification (AO/PI Dual Fluorescence Method).
In addition to trypan blue staining, AO/PI dual fluorescence staining has become popular as a more accurate live cell counting method and is widely used for primary cell counting.

FDA 21 CFR Part11 compliance

Ensuring data security with account login and account permissions setting.

Ensuring data integrity with electronic signature and electronic recording.

Parameters	Description
Model	C100-Pro
Optional fluorescence application	（Ex ：375/28nm ；Em：460/50nm） （Ex ：480/30nm ；Em：535/40nm） （Ex ：540/25nm ；Em：620/60nm ）
Amplification Factor	2.5×, 5.0 Megapixel
Focus Method	Manual focusing & Autofocusing
Counting Area	2.15 mm×1.62 mm
Cell Type	Cell lines, stem cells, primary cells, pollens, beer yeast
Cell Size Range	4-60 μm (Optimal: 7-60 μm)
Cell Concentration Range	10^4-10^7 cells/mL
Cell counting Time	Less than 9s
Historical Data Storage	1000 counting reports and images at most
Languages	Chinese and English
Dimensions	212 mm(W)* 264 mm(H)* 165 mm (D)
Weight	~3.5 kg

 

FL Cube	Excitation	Emission	General Dyes
FL Cube-01	375/28nm	460/50nm	DAPI，Hoechst，BFP
FL Cube-02	480/30nm	535/40nm	Acridine Orange（AO），Calcein-AM，Alexa fluor 488，SYBR Green，FITC，GFP
FL Cube-03	540/25nm	620/60nm	Propidium Iodide（PI），DsRed，dTomato，RFP

 

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